(Considering this is a Flash series I thought it wouldn't fit in the Videos thread so tell me to the context the post over if you think differently)

This is perhaps one of the best Game/Anime parody series I have ever seen, if not the best use of Flash and game sprites.

The series is essentially a mix of the Mario Bros games (hence the title), and Sonic the Hedgehog games, with Dragon Ball Z like plot and themes for extra flavouring. What results is a great spoof of the games and anime shows in general (particularly the action genre shows); you don't even have to be a particular fan of any of the aforementioned games/show to enjoy it (though you might get more of the references).

Here are the episodes so far:
Episode 1: http://www.newgrounds.com/portal/view/308690
Episode 2: http://www.newgrounds.com/portal/view/314226
Episode 3: http://www.newgrounds.com/portal/view/323940
Episode 4: http://www.newgrounds.com/portal/view/339499
Episode 5: http://www.newgrounds.com/portal/view/363800
Episode 6: http://www.newgrounds.com/portal/view/380713

And to top it off it even has a classic anime-ish opening to go with it:
http://www.newgrounds.com/portal/view/386658

(Note: Although it is from Newgrounds, the series it isn't immature or overly violent like the other flashes the site is known for, so leave the stereotypes at the door. Also it may be recommended to watch this with the latest version of Flash installed.) 

Enjoy!